GLUCOSE-DEPENDENT AND ADPGLC-DEPENDENT STARCH SYNTHESIS IN ISOLATED CAULIFLOWER-BUD AMYLOPLASTS - ANALYSIS OF THE INTERACTION OF VARIOUS POTENTIAL PRECURSORS

被引:13
作者
BATZ, O [1 ]
MAASS, U [1 ]
HENRICHS, G [1 ]
SCHEIBE, R [1 ]
NEUHAUS, HE [1 ]
机构
[1] UNIV OSNABRUCK,FACHBEREICH BIOL CHEM,D-49069 OSNABRUCK,GERMANY
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1994年 / 1200卷 / 02期
关键词
CAULIFLOWER BUD; STARCH SYNTHESIS; GLUCOSE; 6-PHOSPHATE; ADPGLUCOSE; (BRASSICA OLERACEA L);
D O I
10.1016/0304-4165(94)90129-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, we have demonstrated that isolated cauliflower-bud amyloplasts incorporate glucose 6-phosphate at high rates into newly synthesized starch (Neuhaus et al. (1993) Plant Physiol. 101, 573-578). Here we have analyzed the incorporation of radioactively labeled glucose and ADPglucose into newly synthesized starch. It could be shown that glucose incorporation into starch exhibits a typical substrate saturation behaviour and is linear with time for at least 40 min. The incorporation of glucose is strongly dependent upon the intactness of the plastids and upon the presence of both, ATP and 3-phosphoglyceric acid. Using 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) (1) we showed that glucose is taken up into isolated cauliflower-bud amyloplasts as the free glucose molecule, rather than as glucose 6-phosphate. Glucose incorporation into newly synthesized starch is strongly inhibited in the presence of low concentrations of glucose 6-phosphate. The radioactively labeled glucose moiety of ADPglucose is also incorporated into starch. This incorporation can be saturated at increased concentrations of ADPglucose. ATP significantly inhibits the incorporation of the glucose moiety of ADPglucose into starch. This inhibition can be reinforced by the additional presence of glucose 6-phosphate. Glucose 6-phosphate-dependent starch synthesis is not strongly inhibited in the presence of glucose or ADPglucose indicating that glucose 6-phosphate is the precursor for starch synthesis in isolated cauliflower-bud amyloplasts.
引用
收藏
页码:148 / 154
页数:7
相关论文
共 19 条
[1]   IDENTIFICATION OF THE PUTATIVE HEXOSE-PHOSPHATE TRANSLOCATOR OF AMYLOPLASTS FROM CAULIFLOWER BUDS [J].
BATZ, O ;
SCHEIBE, R ;
NEUHAUS, HE .
BIOCHEMICAL JOURNAL, 1993, 294 :15-17
[2]   SPECIFIC TRANSPORT OF INORGANIC-PHOSPHATE, GLUCOSE-6-PHOSPHATE, DIHYDROXYACETONE PHOSPHATE AND 3-PHOSPHOGLYCERATE INTO AMYLOPLASTS FROM PEA ROOTS [J].
BORCHERT, S ;
GROSSE, H ;
HELDT, HW .
FEBS LETTERS, 1989, 253 (1-2) :183-186
[3]   ENZYME-ACTIVITIES ASSOCIATED WITH MAIZE KERNEL AMYLOPLASTS [J].
ECHEVERRIA, E ;
BOYER, CD ;
THOMAS, PA ;
LIU, KC ;
SHANNON, JC .
PLANT PHYSIOLOGY, 1988, 86 (03) :786-792
[4]   LACK OF FRUCTOSE-1,6-BISPHOSPHATASE IN A RANGE OF HIGHER-PLANTS THAT STORE STARCH [J].
ENTWISTLE, G ;
APREES, T .
BIOCHEMICAL JOURNAL, 1990, 271 (02) :467-472
[5]  
HATZFELD WD, 1990, PLANTA, V180, P198, DOI 10.1007/BF00193996
[6]  
HILL LM, 1991, PLANTA, V185, P91, DOI 10.1007/BF00194519
[7]   ENZYMIC CAPACITIES OF PURIFIED CAULIFLOWER BUD PLASTIDS FOR LIPID-SYNTHESIS AND CARBOHYDRATE-METABOLISM [J].
JOURNET, EP ;
DOUCE, R .
PLANT PHYSIOLOGY, 1985, 79 (02) :458-467
[8]   STARCH BIOSYNTHESIS IN DEVELOPING WHEAT-GRAIN - EVIDENCE AGAINST THE DIRECT INVOLVEMENT OF TRIOSE PHOSPHATES IN THE METABOLIC PATHWAY [J].
KEELING, PL ;
WOOD, JR ;
TYSON, RH ;
BRIDGES, IG .
PLANT PHYSIOLOGY, 1988, 87 (02) :311-319
[9]   EFFECT OF TEMPERATURE ON STARCH SYNTHESIS IN POTATO-TUBER TISSUE AND IN AMYLOPLASTS [J].
MOHABIR, G ;
JOHN, P .
PLANT PHYSIOLOGY, 1988, 88 (04) :1222-1228
[10]   PURIFICATION OF HIGHLY INTACT PLASTIDS FROM VARIOUS HETEROTROPHIC PLANT-TISSUES - ANALYSIS OF ENZYMATIC EQUIPMENT AND PRECURSOR DEPENDENCY FOR STARCH BIOSYNTHESIS [J].
NEUHAUS, HE ;
BATZ, O ;
THOM, E ;
SCHEIBE, R .
BIOCHEMICAL JOURNAL, 1993, 296 :395-401