IDENTIFICATION OF THE PUTATIVE HEXOSE-PHOSPHATE TRANSLOCATOR OF AMYLOPLASTS FROM CAULIFLOWER BUDS

被引：19

作者：

BATZ, O ^{[1
]}

SCHEIBE, R ^{[1
]}

NEUHAUS, HE ^{[1
]}

机构：

[1] UNIV OSNABRUCK, FACHBEREICH BIOL CHEM, BARBARASTR 11, D-49069 OSNABRUCK, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1993年 / 294卷

关键词：

D O I：

10.1042/bj2940015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [H-3]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexose-phosphate translocator.

引用

页码：15 / 17

页数：3

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