IODOACETAMIDE TREATMENT OF BOVINE LEUKEMIA-VIRUS GLYCOPROTEIN GP51 ENHANCES THE WESTERN BLOTTING REACTIVITY OF ANTIPEPTIDE ANTIBODIES

被引：5

作者：

CALLEBAUT, I ^{[1
]}

BURNY, A ^{[1
]}

PORTETELLE, D ^{[1
]}

机构：

[1] FAC AGRON GEMBLOUX,PASSAGE DEPORTES 2,B-5030 GEMBLOUX,BELGIUM

来源：

FEBS LETTERS | 1991年 / 292卷 / 1-2期

关键词：

IODOACETAMIDE; BOVINE LEUKEMIA VIRUS; WESTERN BLOTTING; ANTIPEPTIDE; GLYCOPROTEIN; ENHANCEMENT;

D O I：

10.1016/0014-5793(91)80854-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit polyclonal antibodies were raised against synthetic peptides of the bovine leukemia virus envelope glycoprotein gp51 and tested against the full size protein by the Western blotting technique. We show that acetylation of gp51 by iodoacetamide either maintains or significantly increases the antigen-antibody reaction and conclude therefrom that the reactive potential of an anti-peptide antibody may require acetylation of the sulfhydryl groups of the blotted protein.

引用

页码：148 / 150

页数：3

共 5 条

[1] MONOCLONAL-ANTIBODIES DEFINE 8 INDEPENDENT ANTIGENIC REGIONS ON THE BOVINE LEUKEMIA-VIRUS (BLV) ENVELOPE GLYCOPROTEIN-GP51
BRUCK, C
MATHOT, S
PORTETELLE, D
BERTE, C
FRANSSEN, JD
HERION, P
BURNY, A
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[2] CALLEBAUT I, 1991, IN PRESS VIROLOGY, V185
[3] IN ANIMALS INFECTED BY BOVINE LEUKEMIA-VIRUS (BLV) ANTIBODIES TO ENVELOPE GLYCOPROTEIN GP51 ARE DIRECTED AGAINST THE CARBOHYDRATE MOIETY
PORTETELLE, D
BRUCK, C
MAMMERICKX, M
BURNY, A
[J]. VIROLOGY, 1980, 105 (01) : 223 - 233
[4] SYNTHETIC PEPTIDES APPROACH TO IDENTIFICATION OF EPITOPES ON BOVINE LEUKEMIA-VIRUS ENVELOPE GLYCOPROTEIN-GP51
PORTETELLE, D
DANDOY, C
BURNY, A
ZAVADA, J
SIAKKOU, H
GRASMASSE, H
DROBECQ, H
TARTAR, A
[J]. VIROLOGY, 1989, 169 (01) : 34 - 41
[5] COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOME OF BOVINE LEUKEMIA-VIRUS - ITS EVOLUTIONARY RELATIONSHIP TO OTHER RETROVIRUSES
SAGATA, N
YASUNAGA, T
TSUZUKUKAWAMURA, J
OHISHI, K
OGAWA, Y
IKAWA, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (03) : 677 - 681

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