HEPATOCYTE AGGREGATE CULTURE TECHNIQUE FOR BIOREACTORS IN HYBRID LIVER SUPPORT SYSTEMS

被引：24

作者：

GERLACH, JC

KLOPPEL, K

MULLER, C

SCHNOY, N

SMITH, MD

NEUHAUS, P

机构：

[1] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,INST KLIN CHEM,BERLIN,GERMANY

[2] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,INST PATHOL,BERLIN,GERMANY

[3] UNIV STRATHCLYDE,BIOENGN UNIT,GLASGOW,SCOTLAND

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1993年 / 16卷 / 12期

关键词：

HEPATOCYTES AGGREGATES; CULTURE MODELS; BIOREACTORS; HYBRID LIVER;

D O I：

10.1177/039139889301601210

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Utilizing a modified culture technique for hepatocytes, a high performance suspension culture is possible in which hepatocytes spontaneously form cell aggregates. The aggregates of 20-100 cells have been histologically confirmed to hold a three-dimensional structure, they show a long-term external metabolism and a survival time comparable with standard adhesion cultures. This technique has several advantages in the construction of large scale bioreactors for hybrid liver support systems.

引用

页码：843 / 846

页数：4

共 8 条

[1] HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY [J].