EVIDENCE FOR SINGLET OXYGEN-INDUCED CROSS-LINKS AND AGGREGATION OF COLLAGEN

被引：21

作者：

KAKEHASHI, A

AKIBA, J

UENO, N

CHAKRABARTI, B

机构：

[1] HARVARD UNIV,SCH MED,SCHEPENS EYE RES INST,20 STANIFORD ST,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT OPHTHALMOL,BOSTON,MA 02115

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Singlet oxygen, generated by a hematoporphyrin-photosensitized reaction, was shown to cause insolubilization and an increase in molecular weight of acid soluble type I collagen and vitreous collagen as manifested in sodium dodecyl sulfate polyacrylamide gel electrophoresis. No such changes in the molecular properties of collagen could be observed when the irradiation was carried out in the presence of sodium azide, a singlet oxygen quencher. The increase in molecular weight and insolubilization of the collagen solution was attributed to extensive cross-links in the protein molecules. © 1993 Academic Press, Inc.

引用

页码：1440 / 1446

页数：7

共 30 条

[21]

Schepens C L, 1987, Bull Soc Belge Ophtalmol, V223 Pt 1, P85

[22] AGE-RELATED-CHANGES IN HUMAN VITREOUS STRUCTURE [J].