PURIFICATION AND BIOCHEMICAL-CHARACTERIZATION OF DOG GASTRIC LIPASE

被引：107

作者：

CARRIERE, F

MOREAU, H

RAPHEL, V

LAUGIER, R

BENICOURT, C

JUNIEN, JL

VERGER, R

机构：

[1] CNRS,CTR BIOCHIM & BIOL MOLEC,BP 71,F-13402 MARSEILLE 09,FRANCE

[2] INSERM,U315,F-13258 MARSEILLE 09,FRANCE

[3] INST RECH JOUVEINAL,FRESNES,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16346.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lipase was found to be present in dog stomach which appeared to be more abundant in the fundic than in the pyloric mucosa. Dog gastric lipase was extracted by soaking the gastric tissue and further purified after cation exchange, anion exchange and gel-filtration using fast protein liquid chromatography. The amino-acid composition, N-terminal amino-acid sequence, substrate specificity, interfacial and kinetic behavior and inactivation by sulfhydryl reagents were determined and compared with those of human and rabbit gastric lipases. We report for the first time that a gastric lipase is 13 times more active on long-chain than on short-chain triacylglycerols at pH 4.0, reaching a maximal specific activity of 950 U/mg on Intralipide emulsion.

引用

页码：75 / 83

页数：9

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