GLUTATHIONE-PEROXIDASE ACTIVITY IN SURGICAL AND AUTOPSIED HUMAN BRAINS

被引：9

作者：

ANSARI, KA

BIGELOW, D

KAPLAN, E

机构：

[1] UNIV MINNESOTA, DEPT NEUROL, MINNEAPOLIS, MN 55455 USA

[2] VET ADM MED CTR, RES SERV, MINNEAPOLIS, MN 55417 USA

[3] UNIV MINNESOTA, DEPT MED, MINNEAPOLIS, MN 55455 USA

来源：

NEUROCHEMICAL RESEARCH | 1985年 / 10卷 / 05期

关键词：

D O I：

10.1007/BF00964408

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathione peroxidase (GSHPx) activity was assayed in normal cerebral gray and white matter samples obtained from frontal, temporal, occipital and parietal lobes during surgical approach to an underlying lesion, and also in normal autopsied human frontal gray and white matter. GSHPx was assayed by a 2 step enzyme reaction which was monitored by following the oxidation of NADPH at 340 nm. All the brain samples studied contained GSHPx activity. Parietal lobe appeared to have the lowest GSHPx activity compared to temporal, occipital or frontal lobes. Mean enzyme activity in autopsied samples was comparable to that in surgical material. Considerable loss of activity was observed after 10 yr of tissue storage at -80.degree. C.

引用

页码：703 / 711

页数：9

共 26 条

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