CONTINUOUS-FLOW STOPPED-FLOW SYSTEM INCORPORATING 2 NOTATING BIOREACTORS IN TANDEM - APPLICATION TO THE DETERMINATION OF ALKALINE-PHOSPHATASE ACTIVITY IN SERUM

被引：11

作者：

RABA, J ^{[1
]}

MOTTOLA, HA ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,DEPT CHEM,STILLWATER,OK 74078

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 09期

关键词：

D O I：

10.1021/ac00081a020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two rotating bioreactors in tandem have been incorporated into a continuous-flow/stopped-flow sample/reagent processing setup for the determination of alkaline phosphatase (EC 3.1.3.1) activity in serum samples. The strategy circumvents incompatibility of buffer systems as well as that of the immobilized enzymes utilized in the bioreactors (alkaline phosphatase and alcohol oxidase, EC 1.1.3.13). The determination is indirect in nature although recorded responses are directly related to the enzyme activity in the sample. It couples the following enzyme-catalyzed reactions: (1) hydrolysis of p-nitrophenyl dihydrogen phosphate catalyzed by alkaline phosphatase, (2) enzymatic reaction between unreacted p-nitrophenyl dihydrogen phosphate with methanol, and (3) conversion of the residual methanol to the corresponding aldehyde and H2O2, catalyzed by alcohol oxidase. The H2O2 is amperometrically determined at a stationary Pt-ring electrode (applied potential + 0.600 V vs a Ag/AgCl, 3.0 M NaCl reference).

引用

页码：1485 / 1489

页数：5

共 7 条

[1] COMPARATIVE-STUDIES ON DATA-COLLECTION AND DATA TREATMENT IN KINETIC-BASED DETERMINATIONS WITH 2 ROTATING BIOREACTOR AMPEROMETRIC DETECTION SYSTEMS [J].