INHERITANCE OF CHROMOSOMAL LENGTH POLYMORPHISMS IN THE ASCOMYCETE LEPTOSPHAERIA-MACULANS

被引：42

作者：

PLUMMER, KM ^{[1
]}

HOWLETT, BJ ^{[1
]}

机构：

[1] UNIV MELBOURNE,SCH BOT,PLANT CELL BIOL RES CTR,PARKVILLE,VIC 3052,AUSTRALIA

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 247卷 / 04期

关键词：

LEPTOSPHAERIA MACULANS; CHROMOSOMAL LENGTH POLYMORPHISMS; MEIOTIC RECOMBINATION; TETRADS;

D O I：

10.1007/BF00293142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pulsed field gel electrophoresis experiments show that chromosomal length polymorphisms are produced during meiosis in the ascomycetous plant pathogen Leptosphaeria maculans. Homologues in tetrads of L, maculans were identified on the basis of their binding to chromosome-specific probes that included P-tubulin, nitrate reductase, 18S ribosomal DNA and two Random Amplified Polymorphic DNA (RAPD) markers. Changes in size of homologues were followed during meiosis. Significant karyotype variation was evident due to the random assortment of parental homologues of different sizes. In most cases, the progeny had the same-sized homologues as the parents; however, in some instances novel-sized homologues were detected that varied in size from those of the parents by up to 50 kb. Our results are consistent with the hypothesis that these novel chromosomal length polymorphisms are produced by reciprocal recombination between parental homologous chromosomes of unequal sizes.

引用

页码：416 / 422

页数：7

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