SEX AND DEVELOPMENT IN BOVINE INVITRO FERTILIZED EMBRYOS

被引：142

作者：

AVERY, B

MADISON, V

GREVE, T

机构：

[1] Department of Reproduction, Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C

来源：

THERIOGENOLOGY | 1991年 / 35卷 / 05期

关键词：

SEXING; CATTLE; INVITRO FERTILIZATION; DIFFERENTIAL GROWTH RATE;

D O I：

10.1016/0093-691X(91)90306-X

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vitro fertilized bovine embryos were karyotyped at Day 7 and Day 8 post insemination. The results showed that the percentage of males (sex ratio) was dependent on the developmental stage. Among expanded blastocysts (the most advanced embryos), the sex ratio was 100%, followed by 89% for expanding blastocysts, 75% for blastocysts, and 40% for young blastocysts, all analyzed at Day 7. For embryos karyotyped at Day 8, the sex ratio was 20%. The average mitotic index for all in vitro fertilized embryos was 3.5%. These results suggest that the apparent relationship between sex and developmental rate could be used as a method for noninvasive sexing of in vitro fertilized embryos.

引用

页码：953 / 963

页数：11