DNA-BINDING ACTIVITY OF RECOMBINANT SRY FROM NORMAL MALES AND XY FEMALES

被引：385

作者：

HARLEY, VR

JACKSON, DI

HEXTALL, PJ

HAWKINS, JR

BERKOVITZ, GD

SOCKANATHAN, S

LOVELLBADGE, R

GOODFELLOW, PN

机构：

[1] IMPERIAL CANC RES FUND,HUMAN MOLEC GENET LAB,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

[2] JOHNS HOPKINS CHILDREN CTR,DIV PEDIAT ENDOCRINOL,BALTIMORE,MD 21205

[3] NATL INST MED RES,MRC,EUKARYOT MOLEC GENET LAB,LONDON NW7 1AA,ENGLAND

来源：

SCIENCE | 1992年 / 255卷 / 5043期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1126/science.1734522

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.

引用

页码：453 / 456

页数：4