SITE-SPECIFIC GLYCOSYLATION OF BOVINE BUTYROPHILIN

被引:45
作者
SATO, T
TAKIO, K
KOBATA, A
GREENWALT, DE
FURUKAWA, K
机构
[1] UNIV TOKYO,INST MED SCI,DEPT BIOCHEM,MINATO KU,TOKYO 108,JAPAN
[2] RIKEN,INST PHYS & CHEM RES,DIV BIOMOLEC CHARACTERIZAT,WAKO,SAITAMA 35101,JAPAN
[3] AMER RED CROSS,JEROME H HOLLAND LAB,ROCKVILLE,MD 20855
关键词
N-ACETYLGALACTOSAMINYLATION; BUTYROPHILIN; N-GLYCOSYLATION; SITE SPECIFICITY;
D O I
10.1093/oxfordjournals.jbchem.a124702
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Our previous studies showed that the N-linked sugar chains of most bovine glycoproteins from milk fat globule membranes (MFGM) contain the GalNAc beta 1-->4GlcNAc group [Sato et al, (1993) J. Biochem, 114, 890-900], Since expression of the disaccharide structure is influenced by peptide sequences near the glycosylation sites [Smith and Baenziger (1992) Proc. Natl. Acad Sci. USA 89, 329-333], the site-specificity of the N-acetylgalactosaminylated sugar chains was investigated using bovine butyrophilin, a major MFGM glycoprotein with known primary structure. Two glycopeptide fragments which contained the N-linked sugar chains linked to either Asn-55 or Asn-215 residue were obtained by digestion of the protein with Achromobacter protease I. The sugar chains released from each glycopeptide by hydrazinolysis were reduced with (NaBH4)-H-3. Structural analyses of the oligosaccharides by sequential exoglycosidase digestion and methylation analysis revealed that only complex-type sugar chains with the GalNAc beta 1-->4GlcNAc structure are included in Asn-55-linked oligosaccharides, while only novel hybrid-type sugar chains detected previously in bovine MFGM glycoproteins are included in Asn-215-linked oligosaccharides. The results show that the glycosylation of butyrophilin occurs in a site-specific manner.
引用
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页码:147 / 157
页数:11
相关论文
共 35 条
[1]   HUMAN UROKINASE CONTAINS GALNAC-BETA(1-4)[FUC-ALPHA(1-3)]GLCNAC-BETA(1-2) AS A NOVEL TERMINAL ELEMENT IN N-LINKED CARBOHYDRATE CHAINS [J].
BERGWERFF, AA ;
THOMASOATES, JE ;
VANOOSTRUM, J ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
FEBS LETTERS, 1992, 314 (03) :389-394
[2]  
CHAN A L, 1991, Glycobiology, V1, P173, DOI 10.1093/glycob/1.2.173
[3]   A HEPATIC RETICULOENDOTHELIAL CELL-RECEPTOR SPECIFIC FOR SO4-4GAINAC-BETA-1,4GLCNAC-BETA-1,2MAN-ALPHA THAT MEDIATES RAPID CLEARANCE OF LUTROPIN [J].
FIETE, D ;
SRIVASTAVA, V ;
HINDSGAUL, O ;
BAENZIGER, JU .
CELL, 1991, 67 (06) :1103-1110
[4]  
FRIEDMAN M, 1970, J BIOL CHEM, V245, P3868
[5]   STRUCTURAL STUDY OF THE SUGAR CHAINS OF HUMAN-PLATELET THROMBOSPONDIN [J].
FURUKAWA, K ;
ROBERTS, DD ;
ENDO, T ;
KOBATA, A .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 270 (01) :302-312
[6]  
GLASGOW LR, 1977, J BIOL CHEM, V252, P8615
[7]  
GREEN ED, 1985, J BIOL CHEM, V260, P5623
[8]  
GREEN ED, 1988, J BIOL CHEM, V263, P25
[9]  
GREEN ED, 1988, J BIOL CHEM, V263, P36
[10]   THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS [J].
HARD, K ;
VANZADELHOFF, G ;
MOONEN, P ;
KAMERLING, JP ;
VLIEGENTHART, JFG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (03) :895-915