SCALED-UP EXPRESSION OF HUMAN ALPHA-2,6(N)SIALYLTRANSFERASE IN SACCHAROMYCES-CEREVISIAE

被引：19

作者：

BORSIG, L ^{[1
]}

IVANOV, SX ^{[1
]}

HERRMANN, GF ^{[1
]}

KRAGL, U ^{[1
]}

WANDREY, C ^{[1
]}

BERGER, EG ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL 2, D-52425 JULICH, GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1621

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of recombinant full length human alpha 2,6(N)sialyltransferase has been scaled-up in S. cerevisiae in a 150-1 bioreactor yielding 47 U at a concentration of 0.31 U/l. The protein specific activity as measured in reconstituted yeast lyophilisate was 0.8 mU/mg protein. The recombinant enzyme exhibited similar Michaelis constants as previously determined for the native rat enzyme. By immunoblotting the enzyme was shown to be heterogeneous by size (44-48 kD) and N-glycosylated. We conclude that recombinant alpha 2,6(N)sialyltransferase expressed in S. cerevisiae is retained in the endoplasmic reticulum as a fully active enzyme. (C) 1995 Academic Press, Inc.

引用

页码：14 / 20

页数：7

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