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DEVELOPMENT AND APPLICATION OF AN IMMOBILIZED ENZYME ELECTRODE FOR THE DETERMINATION OF SULFITE IN FOODS AND FEEDS

被引：11
作者：
NABIRAHNI, MA
VAID, RR
机构：
[1] Department of Chemistry & Physical Sciences, Pace University
来源：
ANALYTICAL LETTERS | 1991年 / 24卷 / 04期
关键词：
ENZYME ELECTRODE; SULFITE DETERMINATION; SULFITE OXIDASE; FOODS AND FEEDS;
D O I：
10.1080/00032719108052926
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An enzyme electrode was developed using the enzyme sulfite oxidase (EC 1.8.3.1) immobilized onto pig intestine. All experimental parameters such as pH, temperature, buffer constituent concentration, etc., were thoroughly investigated and optimized when appropriate. Hydrogen peroxide was monitored amperometrically. The response to sulfite concentration was linear in the range of 5.2x10(-6) to 1.0x10(-3)M. The proposed method was found to have a correlation coefficient of 0.952 when evaluated versus the standard titration method.
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页码：551 / 565
页数：15
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