CONSTITUTIVE EXPRESSION OF C-FOS GENE INHIBITS TYPE-1 COLLAGEN-SYNTHESIS IN TRANSFECTED OSTEOBLASTS

被引：28

作者：

KUROKI, Y

SHIOZAWA, S

SUGIMOTO, T

FUJITA, T

机构：

[1] Department of Medicine, Third Division, Kobe University School of Medicine, Kobe

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91887-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the contribution of c-fos DNA to bone formation, the effect of constitutive expression of the c-fos gene in collagen synthesis was examined by introducing c-fos DNA into osteoblastic MC3T3-E1 cells. The [3H] proline incorporation into the collagenase digestible protein(CDP) and the percent collagen synthesis were significantly decreased in the c-fos transfectants which constitutively express c-fos mRNA as compared with control transfectants. Transcription of type 1(α1) collagen gene was also specifically decreased in the c-fos transfectants. This indicates that constitutive expression of c-fos DNA interferes with bone formation by inhibiting collagen synthesis in osteoblasts. © 1992.

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页码：1389 / 1394

页数：6

相关论文

共 21 条

[11] REMOVAL OF A 67-BASE-PAIR SEQUENCE IN THE NONCODING REGION OF PROTOONCOGENE-FOS CONVERTS IT TO A TRANSFORMING GENE [J].

MEIJLINK, F ;

CURRAN, T ;

MILLER, AD ;

VERMA, IM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :4987-4991

[12] RAPID INDUCTION OF THE EXPRESSION OF PROTO-ONCOGENE FOS DURING HUMAN MONOCYTIC DIFFERENTIATION [J].

MITCHELL, RL ;

ZOKAS, L ;

SCHREIBER, RD ;

VERMA, IM .

CELL, 1985, 40 (01) :209-217

[13] DIFFERENTIATION OF F9 TERATOCARCINOMA STEM-CELLS AFTER TRANSFER OF C-FOS PROTO-ONCOGENES [J].

MULLER, R ;

WAGNER, EF .

NATURE, 1984, 311 (5985) :438-442

[14] DIFFERENTIAL EXPRESSION OF C-FOS IN HEMATOPOIETIC-CELLS - CORRELATION WITH DIFFERENTIATION OF MONOMYELOCYTIC CELLS-INVITRO [J].

MULLER, R ;

MULLER, D ;

GUILBERT, L .

EMBO JOURNAL, 1984, 3 (08) :1887-1890

[15] A CDNA CLONING VECTOR THAT PERMITS EXPRESSION OF CDNA INSERTS IN MAMMALIAN-CELLS [J].

OKAYAMA, H ;

BERG, P .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (02) :280-289

[16] DEREGULATED C-FOS EXPRESSION INTERFERES WITH NORMAL BONE-DEVELOPMENT IN TRANSGENIC MICE [J].

RUTHER, U ;

GARBER, C ;

KOMITOWSKI, D ;

MULLER, R ;

WAGNER, EF .

NATURE, 1987, 325 (6103) :412-416

[17]

SANDMEYER S, 1981, J BIOL CHEM, V256, P5022

[18] REGULATION OF A COLLAGEN GENE PROMOTER BY THE PRODUCT OF VIRAL MOS ONCOGENE [J].

SCHMIDT, A ;

SETOYAMA, C ;

DECROMBRUGGHE, B .

NATURE, 1985, 314 (6008) :286-289

[19] REQUIREMENT FOR FOS GENE-EXPRESSION IN THE TRANSCRIPTIONAL ACTIVATION OF COLLAGENASE BY OTHER ONCOGENES AND PHORBOL ESTERS [J].

SCHONTHAL, A ;

HERRLICH, P ;

RAHMSDORF, HJ ;

PONTA, H .

CELL, 1988, 54 (03) :325-334

[20] REGULATION OF PROCOLLAGEN MESSENGER RIBONUCLEIC-ACID LEVELS IN ROUS-SARCOMA VIRUS-TRANSFORMED CHICK-EMBRYO FIBROBLASTS [J].

SOBEL, ME ;

YAMAMOTO, T ;

DECROMBRUGGHE, B ;

PASTAN, I .

BIOCHEMISTRY, 1981, 20 (09) :2678-2684