METHOD FOR DETERMINING PARACETAMOL IN WHOLE-BLOOD BY CHRONOAMPEROMETRY FOLLOWING ENZYMATIC-HYDROLYSIS

被引：24

作者：

BRAMWELL, H

CASS, AEG

GIBBS, PNB

GREEN, MJ

机构：

[1] IMPERIAL COLL SCI TECHNOL & MED,CTR BIOTECHNOL,LONDON SW7 2AZ,ENGLAND

[2] MEDISENSE UK INC,ABINGDON OX14 1DY,OXON,ENGLAND

来源：

ANALYST | 1990年 / 115卷 / 02期

关键词：

Arylacylamide amidohydrolase; Chronoamperometry; P-aminophenol; Paracetamol;

D O I：

10.1039/an9901500185

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is proposed for the determination of paracetamol in whole undiluted blood, based on the enzymatic hydrolysis of the drug to p-aminophenol, which is then measured by chronoamperometry at a glassy carbon electrode. Hydrolysis of the paracetamol prior to electro-oxidation is shown to alleviate problems that arise from high background currents in the whole blood and so ensures a good linear correlation (r>0.99) between the current and the paracetamol concentration. Recovery experiments and comparison with a reference method based on spectrophotometry suggest that the electrochemical assay only measures that proportion of paracetamol that is not bound to serum albumin.

引用

页码：185 / 188

页数：4