HISTOCHEMICAL AND IMMUNOCHEMICAL STUDY OF THE FATE OF CANDIDA-ALBICANS INSIDE HUMAN NEUTROPHIL PHAGOLYSOSOMES

被引:15
作者
MARQUIS, G [1 ]
GARZON, S [1 ]
MONTPLAISIR, S [1 ]
STRYKOWSKI, H [1 ]
BENHAMOU, N [1 ]
机构
[1] UNIV LAVAL, DEPT PHYTOL, Ste Foy, QUEBEC, CANADA
关键词
GRANULOCYTE; PHAGOSOME; IMMUNOGOLD; CYTOCHEMISTRY; ULTRASTRUCTURE;
D O I
10.1002/jlb.50.6.587
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To further define the ultrastructural events associated with the killing of Candida albicans by human neutrophils, four methods were used: (1) the periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining of vicinal-glycol-containing complex carbohydrates; (2) the localization of thermostable immunodeterminants of the yeast cell wall, mannans or mannoproteins, using monospecific antibodies and a protein A-gold complex (monAb-gold); (3) the localization of mannose residues with concanavalin A labeled with gold particles (Con A-gold); (4) the localization of chitin oligomers using wheat germ agglutinin and ovomucoid labeled with gold particles (WGA-gold). The mannan-rich cell wall layers were progressively lost as shown by altered PA-TCH-SP reactivity and a diffuse pattern of staining with Con A-gold and monAb-gold. The de novo appearance of conspicuous amounts of glycogen-like particles near the plasmalemma and in the cell wall was interpreted as evidence of a reparative process of the yeast cell wall. Chitin was seemingly unaltered and readily demonstrated by the WGA-gold in the wall remnants of ghost cells.
引用
收藏
页码:587 / 599
页数:13
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