MICROBATCH CRYSTALLIZATION UNDER OIL - A NEW TECHNIQUE ALLOWING MANY SMALL-VOLUME CRYSTALLIZATION TRIALS

被引：210

作者：

CHAYEN, NE ^{[1
]}

STEWART, PDS ^{[1
]}

BLOW, DM ^{[1
]}

机构：

[1] DOUGLAS INSTRUMENTS LTD,LONDON SW11 4NB,ENGLAND

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0022-0248(92)90241-A

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

An approach to rapid protein crystallization using very small samples is described. A computer controlled microdispenser is used to make crystallization samples as microbatch droplets under oil. Samples of 1-2 mul are dispensed ready-mixed and with good precision. The samples are protected from evaporation, contamination and physical shock by the oil. When favourable conditions for crystallization have been found using one mode of the system, the size and quantity of crystals are optimized by a second program which generates a set of conditions throughout the area of interest. Crystals of diffraction size and quality have been grown in 1 mul drops.

引用

页码：176 / 180

页数：5

共 10 条

[1]

CARTER CW, 1979, J BIOL CHEM, V254, P2219

[2] AN AUTOMATED-SYSTEM FOR MICROBATCH PROTEIN CRYSTALLIZATION AND SCREENING [J].