DETECTION OF HEPATITIS-C VIRUS CDNA SEQUENCE BY THE POLYMERASE CHAIN-REACTION IN HEPATOCELLULAR-CARCINOMA TISSUES

被引：20

作者：

YONEYAMA, T

TAKEUCHI, K

WATANABE, Y

HARADA, H

OHBAYASHI, A

TANAKA, Y

YUASA, T

SAITO, I

MIYAMURA, T

机构：

[1] ST MARIANNA MED UNIV,SCH MED,DEPT INTERNAL MED 2,MIYAMAE KU,KAWASAKI,KANAGAWA 213,JAPAN

[2] NATL TOKYO SANATORIUM HOSP,DEPT GASTROENTEROL,LIVER UNIT,TOKYO 204,JAPAN

来源：

JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY | 1990年 / 43卷 / 03期

关键词：

D O I：

10.7883/yoken1952.43.89

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We found the presence of hepatitis C virus (HCV) infection in liver tissues of hepatocellular carcinoma (HCC) patients who had antibodies to HCV but no serological markers for hepatitis B virus infection by the sensitive reverse transcription/polymerase chain reaction (R/PCR) method. The primers used were derived from the non-structural (NS) 3 and/or the structural (C/E) region. Amplified cDNA sequences of HCV were detected in either cancerous or noncancerous portion of liver tissues from four out of eight HCC patients with primers of NS3 region. Similar but less efficient results were obtained with primers of C/E region. These results indicate that HCV persists in the liver tissue of HCC. A possible role of persistent infection of HCV for the development of HCC is discussed. © 1990, National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee. All rights reserved.

引用

页码：89 / 94

页数：6

共 16 条

[1] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].

ALTER, HJ ;

PURCELL, RH ;

SHIH, JW ;

MELPOLDER, JC ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500

[2] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[3]

COLOMBO M, 1989, LANCET, V2, P1006

[4] BLOOD SCREENING FOR NON-A, NON-B HEPATITIS BY HEPATITIS-C VIRUS-ANTIBODY ASSAY [J].

KATAYAMA, T ;

KIKUCHI, S ;

TANAKA, Y ;

SAITO, I ;

MIYAMURA, T ;

CHOO, QL ;

HOUGHTON, M ;

KUO, G .

TRANSFUSION, 1990, 30 (04) :374-376

[5] A CDNA FRAGMENT OF HEPATITIS-C VIRUS ISOLATED FROM AN IMPLICATED DONOR OF POST-TRANSFUSION NON-A, NON-B HEPATITIS IN JAPAN [J].

KUBO, Y ;

TAKEUCHI, K ;

BOONMAR, S ;

KATAYAMA, T ;

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

BRADLEY, DW ;

HOUGHTON, M ;

SAITO, I ;

MIYAMURA, T .

NUCLEIC ACIDS RESEARCH, 1989, 17 (24) :10367-10372

[6] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].

KUO, G ;

CHOO, QL ;

ALTER, HJ ;

GITNICK, GL ;

REDEKER, AG ;

PURCELL, RH ;

MIYAMURA, T ;

DIENSTAG, JL ;

ALTER, MJ ;

STEVENS, CE ;

TEGTMEIER, GE ;

BONINO, F ;

COLOMBO, M ;

LEE, WS ;

KUO, C ;

BERGER, K ;

SHUSTER, JR ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :362-364

[7] DETECTION OF ANTIBODY AGAINST ANTIGEN EXPRESSED BY MOLECULARLY CLONED HEPATITIS-C VIRUS CDNA - APPLICATION TO DIAGNOSIS AND BLOOD SCREENING FOR POSTTRANSFUSION HEPATITIS [J].

MIYAMURA, T ;

SAITO, I ;

KATAYAMA, T ;

KIKUCHI, S ;

TATEDA, A ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :983-987

[8]

MUCHMORE E, 1988, J MED PRIMATOL, V17, P235

[9]

OHTA Y, 1976, HEPATOCELLULAR CARCI, P73

[10]

OKAYAMA H, 1987, METHOD ENZYMOL, V154, P3

← 1 2 →