MOLECULAR CHARACTERIZATION OF SPECIFIC-HEAT SHOCK PROTEINS IN BACILLUS-SUBTILIS

被引：32

作者：

MILLER, BS

KENNEDY, TE

STREIPS, UN

机构：

[1] UNIV LOUISVILLE,SCH MED,DEPT MICROBIOL & IMMUNOL,LOUISVILLE,KY 40292

[2] COLUMBIA UNIV,CTR NEUROBIOL & BEHAV,NEW YORK,NY 10027

来源：

CURRENT MICROBIOLOGY | 1991年 / 22卷 / 04期

关键词：

D O I：

10.1007/BF02092314

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Heat shock in Bacillus subtilis may induce as many as 66 proteins after temperature upshift from 37-degrees to 48-degrees-C. Four induced proteins were analyzed by microsequencing techniques. These were identified as the homologues for GroEL, DnaK, enolase, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which are heat shock proteins in other systems. The identities of GroEL and DnaK were confirmed additionally by Western blot analysis. As a control, a protein whose synthesis was repressed approximately threefold by heat shock was identified by microsequencing as flagellin.

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页码：231 / 236

页数：6

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