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MOLECULAR CHARACTERIZATION OF SPECIFIC-HEAT SHOCK PROTEINS IN BACILLUS-SUBTILIS

被引：32
作者：
MILLER, BS
KENNEDY, TE
STREIPS, UN
机构：
[1] UNIV LOUISVILLE,SCH MED,DEPT MICROBIOL & IMMUNOL,LOUISVILLE,KY 40292
[2] COLUMBIA UNIV,CTR NEUROBIOL & BEHAV,NEW YORK,NY 10027
来源：
CURRENT MICROBIOLOGY | 1991年 / 22卷 / 04期
关键词：
D O I：
10.1007/BF02092314
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Heat shock in Bacillus subtilis may induce as many as 66 proteins after temperature upshift from 37-degrees to 48-degrees-C. Four induced proteins were analyzed by microsequencing techniques. These were identified as the homologues for GroEL, DnaK, enolase, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which are heat shock proteins in other systems. The identities of GroEL and DnaK were confirmed additionally by Western blot analysis. As a control, a protein whose synthesis was repressed approximately threefold by heat shock was identified by microsequencing as flagellin.
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页码：231 / 236
页数：6
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