INTERNUCLEOSOMAL CHROMATIN DEGRADATION IN MYELOMA AND B-HYBRIDOMA CELL-CULTURES

被引：11

作者：

SOKOLOVA, IA ^{[1
]}

VOLGIN, AU ^{[1
]}

MAKAROVA, NV ^{[1
]}

VOLGINA, VV ^{[1
]}

SHISHKIN, SS ^{[1
]}

KHODAREV, NN ^{[1
]}

机构：

[1] RUSSIAN MINIST HLTH,CTR MED BIOTECHNOL,MOSCOW,USSR

来源：

FEBS LETTERS | 1992年 / 313卷 / 03期

关键词：

HYBRIDOMA; APOPTOSIS; CA/MG-DEPENDENT ENDONUCLEASE;

D O I：

10.1016/0014-5793(92)81213-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c as compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME. which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time-course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin.

引用

页码：295 / 299

页数：5

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