AN IMPROVED MICRO ENZYME SENSOR FOR BIOPROCESS MONITORING BY FLOW-INJECTION ANALYSIS

An improved micro enzyme electrode is presented for on-line determination of glucose during bioprocesses. The disposable sensor was constructed on the basis of an integrated microelectrode (from the Institute of Microtechnique, University of Neuchatel) and used as detector in a flow injection analysis (FIA) system. Glucose oxidase was incorporated in a polyvinyl pyridine matrix doped with mediator molecules and deposited on the electrode. The performance of this sensor was tested during the cultivation of yeast. The obtained results were compared with the data from a different flow injection method. using enzyme columns and colorimetric detection.