EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF DEFLAZACORT AND ITS METABOLITE 21-HYDROXYDEFLAZACORT - APPLICATION TO URINE SAMPLES

被引：12

作者：

SANTOSMONTES, A ^{[1
]}

GONZALOLUMBRERAS, R ^{[1
]}

GASCOLOPEZ, AI ^{[1
]}

IZQUIERDOHORNILLOS, R ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC QUIM,DEPT QUIM ANAL,E-28040 MADRID,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)80096-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Two HPLC methods for the separation of a mixture of corticoids including the oxazolinic corticoid deflazacort and its metabolite 21-hydroxydeflazacort using different water-tetrahydrofuran mobile phases were developed. Both separations allowed the detection and determination of fifteen out of sixteen corticoids using different C,, columns. Extraction data for deflazacort and its metabolite using different extraction procedures are also reported. These separation conditions were applied to urine samples from two male volunteers administered Dezacor, with both doping control and clinical purposes.

引用

页码：248 / 253

页数：6

共 5 条

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[2]

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[3]

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SANTOSMONTES, A ;

GASCOLOPEZ, AI ;

IZQUIERDOHORNILLOS, R .

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[4]

SOLVENT AND SOLID-PHASE EXTRACTION OF NATURAL AND SYNTHETIC CORTICOIDS IN HUMAN URINE [J].

SANTOSMONTES, A ;

GONZALOLUMBRERAS, R ;

GASCOLOPEZ, AI ;

IZQUIERDOHORNILLOS, R .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 652 (01) :83-89

[5]

SCHIATTI P, 1980, DRUG RES, V30, P15434

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