GLUCOSE REGULATES THE EXPRESSION OF GI-PROTEINS IN CULTURED BC3H-1 MYOCYTES

被引：5

作者：

MOISES, RS

HEIDENREICH, KA

机构：

[1] Department of Medicine, Division of Endocrinology and Metabolism, University of California, San Diego, La Jolla

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91858-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To examine whether glucose has regulatory effects on the expression of Gi-proteins, BC3H-1 myocytes were incubated for 24 hr in the presence of various concentrations of glucose (0-25 mM) and the amount of Gi-proteins was detected by pertussis toxin ADP-ribosylation and immunoblot analysis. Both detection methods showed a progressive decrease in the amount of Gi proteins in cells treated with increasing concentrations of glucose. A maximal reduction of 40% was observed after a 24 hr exposure to 25 mM glucose. The reduction in Gi-proteins correlated with a decrease in insulin-stimulated glucose transport. © 1992.

引用

页码：1193 / 1200

页数：8

共 13 条

[1] ABOLITION OF THE EXPRESSION OF INHIBITORY GUANINE-NUCLEOTIDE REGULATORY PROTEIN-GI ACTIVITY IN DIABETES [J].

GAWLER, D ;

MILLIGAN, G ;

SPIEGEL, AM ;

UNSON, CG ;

HOUSLAY, MD .

NATURE, 1987, 327 (6119) :229-232

[2]

GILMAN AG, 1987, ANNU REV BIOCHEM, V56, P615, DOI 10.1146/annurev.bi.56.070187.003151

[3] ACTION OF INSULIN MODULATED BY PERTUSSIS TOXIN IN RAT ADIPOCYTES [J].

GOREN, HJ ;

NORTHUP, JK ;

HOLLENBERG, MD .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1985, 63 (08) :1017-1022

[4] STRUCTURAL AND FUNCTIONAL-CHARACTERISTICS OF INSULIN-RECEPTORS IN RAT NEURO-BLASTOMA CELLS [J].

HEIDENREICH, KA ;

GILMORE, PR .

JOURNAL OF NEUROCHEMISTRY, 1985, 45 (05) :1642-1648

[5]

LUTTRELL LM, 1988, J BIOL CHEM, V263, P6134

[6] PERTUSSIS TOXIN CATALYZED ADP-RIBOSYLATION OF A 41-KDA G-PROTEIN IMPAIRS INSULIN-STIMULATED GLUCOSE-METABOLISM IN BC3H-1 MYOCYTES [J].

MOISES, RS ;

HEIDENREICH, KA .

JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 144 (03) :538-545

[7]

OBRIEN R, 1987, FEBS LETT, V212, P15546

[8] IDENTIFICATION OF THE STEREOSPECIFIC HEXOSE TRANSPORTER FROM STARVED AND FED CHICKEN-EMBRYO FIBROBLASTS [J].

PESSIN, JE ;

TILLOTSON, LG ;

YAMADA, K ;

GITOMER, W ;

CARTERSU, C ;

MORA, R ;

ISSELBACHER, KJ ;

CZECH, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (07) :2286-2290

[9]

ROTHENBERG PL, 1988, J BIOL CHEM, V263, P15546

[10]

SPIEGEL A, 1988, RECENT PROG HORM RES, V44, P337

← 1 2 →