INVESTIGATIONS INTO THE POSTTRANSLATIONAL MODIFICATION AND MECHANISM OF ISOPENICILLIN N-ACYL-COA ACYLTRANSFERASE USING ELECTROSPRAY MASS-SPECTROMETRY

被引：19

作者：

APLIN, RT ^{[1
]}

BALDWIN, JE ^{[1
]}

ROACH, PL ^{[1
]}

ROBINSON, CV ^{[1
]}

SCHOFIELD, CJ ^{[1
]}

机构：

[1] OXFORD CTR MOLEC SCI, OXFORD OX1 3QY, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 294卷

关键词：

D O I：

10.1042/bj2940357

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrospray mass spectrometry (e.s.m.s.) was used to confirm the position of the post-translational cleavage of the isopenicillin N: acyl-CoA acyltransferase preprotein to give the alpha- and beta-subunits. The e.s.m.s. studies suggested partial modification of the alpha-subunit in vivo by exogenously added substituted acetic acids. E.s.m.s. has also allowed the observation in vitro of the transfer of the acyl group from several acyl-CoAs to the beta-subunit. N.m.r. data for the CoA species have been deposited as Supplementary Publication SUP 500173 (2 pages) at the British Library Document Supply Centre (DSC), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, from whom copies can be obtained on the terms indicated in Biochem. J. (1993) 289, 9.

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页码：357 / 363

页数：7

相关论文

共 21 条

[1] ENZYMATIC-SYNTHESIS OF PENICILLINS [J].

ALONSO, MJ ;

BERMEJO, F ;

REGLERO, A ;

FERNANDEZCANON, JM ;

DEBUITRAGO, GG ;

LUENGO, JM .

JOURNAL OF ANTIBIOTICS, 1988, 41 (08) :1074-1084

[2] PURIFICATION TO HOMOGENEITY AND CHARACTERIZATION OF ACYL COENZYME-A-6-AMINOPENICILLANIC ACID ACYLTRANSFERASE OF PENICILLIUM-CHRYSOGENUM [J].

ALVAREZ, E ;

CANTORAL, JM ;

BARREDO, JL ;

DIEZ, B ;

MARTIN, JF .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (11) :1675-1682

[3] USE OF ELECTROSPRAY MASS-SPECTROMETRY TO DIRECTLY OBSERVE AN ACYL ENZYME INTERMEDIATE IN BETA-LACTAMASE CATALYSIS [J].

APLIN, RT ;

BALDWIN, JE ;

SCHOFIELD, CJ ;

WALEY, SG .

FEBS LETTERS, 1990, 277 (1-2) :212-214

[4] STUDIES ON THE INHIBITION OF PORCINE PANCREATIC ELASTASE USING ELECTROSPRAY MASS-SPECTROMETRY [J].

APLIN, RT ;

ROBINSON, CV ;

SCHOFIELD, CJ ;

WESTWOOD, NJ .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1992, (22) :1650-1652

[5] ON THE PRODUCTION OF ALPHA,BETA-HETERODIMERIC ACYL-COENZYME-A - ISOPENICILLIN N-ACYLTRANSFERASE OF PENICILLIUM-CHRYSOGENUM - STUDIES USING A RECOMBINANT SOURCE [J].

APLIN, RT ;

BALDWIN, JE ;

COLE, SCJ ;

SUTHERLAND, JD ;

TOBIN, MB .

FEBS LETTERS, 1993, 319 (1-2) :166-170

[6] SOME ELECTROSPRAY MASS-SPECTROMETRIC EVIDENCE FOR THE EXISTENCE OF COVALENT O-ACYL ENZYME INTERMEDIATES [J].

ASHTON, DS ;

BEDDELL, CR ;

COOPER, DJ ;

GREEN, BN ;

OLIVER, RWA ;

WELHAM, KJ .

FEBS LETTERS, 1991, 292 (1-2) :201-204

[7] CLONING AND CHARACTERIZATION OF THE ACYL-COENZYME-A-6-AMINOPENICILLANIC-ACID-ACYLTRANSFERASE GENE OF PENICILLIUM-CHRYSOGENUM [J].

BARREDO, JL ;

VANSOLINGEN, P ;

DIEZ, B ;

ALVAREZ, E ;

CANTORAL, JM ;

KATTEVILDER, A ;

SMAAL, EB ;

GROENEN, MAM ;

VEENSTRA, AE ;

MARTIN, JF .

GENE, 1989, 83 (02) :291-300

[8]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[9] USE OF ELECTROSPRAY MASS-SPECTROMETRY TO ASSAY CHEMICAL-REACTIVITY OF AN ACYL CARRIER PROTEIN INVOLVED IN FATTY-ACID BIOSYNTHESIS IN SACCHAROPOLYSPORA-ERYTHRAEA [J].

BRIDGES, AM ;

LEADLAY, PF ;

REVILL, WP ;

STAUNTON, J .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1991, (11) :776-777

[10]

COLE M, 1966, PROCESS BIOCHEM, P334