SECE-DEPENDENT OVERPRODUCTION OF SECY IN ESCHERICHIA-COLI - EVIDENCE FOR INTERACTION BETWEEN 2 COMPONENTS OF THE SECRETORY MACHINERY

被引：110

作者：

MATSUYAMA, S

AKIMARU, J

MIZUSHIMA, S

机构：

[1] Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

Escherichia coli; Protein secretion; SecE protein; SecY protein; SecY/SecE interaction;

D O I：

10.1016/0014-5793(90)81128-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-β-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY. The simultaneous induced expression of both genes in the same cells resulted in the overproduction of SecY together with that of SecE. SecY and SecE thus overproduced were localized in the cytoplasmic membrane as those expressed at the normal levels were. It is suggested that SecY and SecE interact with each other in the cytoplasmic membrane. The numbers of the SecY and SecE molecules per cell were estimated. © 1990.

引用

页码：96 / 100

页数：5

共 27 条

[11] A TEMPERATURE-SENSITIVE MUTANT OF ESCHERICHIA-COLI EXHIBITING SLOW PROCESSING OF EXPORTED PROTEINS [J].