GEL PURIFICATION OF RED ALGAL GENOMIC DNA - AN INEXPENSIVE AND RAPID METHOD FOR THE ISOLATION OF POLYMERASE CHAIN REACTION-FRIENDLY DNA

被引：204

作者：

SAUNDERS, GW

机构：

[1] School of Botany, University of Melbourne, Parkville, Victoria

来源：

JOURNAL OF PHYCOLOGY | 1993年 / 29卷 / 02期

关键词：

DNA ISOLATION; POLYMERASE CHAIN REACTION; RHODOPHYTA;

D O I：

10.1111/j.0022-3646.1993.00251.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simplified approach for the extraction of DNA from red algae is presented. Procedures are simple and fast, requiring a minimum of reagents and apparatus. The method involves an initial lysis step followed by an optional phenol/chloroform extraction. The final gel-purification step removes polymerase chain reaction-inhibiting polysaccharides from the DNA preparation. DNA is extracted as easily from dried algae as it is from snap-frozen, fresh material, thus greatly facilitating the collection and transport of algal samples.

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页码：251 / 254

页数：4

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