REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THE STEREOISOMERS OF SOME SWEETENER PEPTIDES WITH A HELICAL NICKEL(II) CHELATE IN THE MOBILE-PHASE

被引：17

作者：

BAZYLAK, G

机构：

[1] Biochromatographic Laboratory, Human Nutrition Division, Hygienics Department, 90 251 Łodz

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 668卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80148-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of a chiral mobile phase additive in the form of the helically distorted, square-planar, chiral nickel(II) chelate dl-[4,4'-(1-methyl-2-propylethane-1,2-diyldiimino)bis(pent-3-en-2-onato)]nickel(II) was investigated for the resolution of optical isomers of dipeptide-type sweeteners, viz., aspartame, alitame and antiaspartame, and some of their decomposition products, e.g., diketopiperazines. The chiral discrimination mechanism for the solutes was elucidated. The proposed chiral RP-HPLC system was applied to the stereoselective determination of aspartame impurities in samples of its commercial dietetic and pharmaceutical formulations.

引用

页码：519 / 527

页数：9

共 23 条

[1] HELICAL NICKEL(II) COMPLEXES AS MOBILE PHASE ADDITIVES IN THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ALKYLAMINE ENANTIOMERS [J].