KINETICS OF CELLOBIOSE HYDROLYSIS USING CELLOBIASE COMPOSITES FROM TRICHODERMA-REESEI AND ASPERGILLUS-NIGER

被引：31

作者：

GROUS, W ^{[1
]}

CONVERSE, A ^{[1
]}

GRETHLEIN, H ^{[1
]}

LYND, L ^{[1
]}

机构：

[1] DARTMOUTH COLL,THAYER SCH ENGN,HANOVER,NH 03755

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1985年 / 27卷 / 04期

关键词：

BIOCHEMISTRY - Mathematical Models - CELLULOSE - Hydrolysis;

D O I：

10.1002/bit.260270411

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic hydrolysis of cellulose to glucose involves the formation of cellobiose as an intermediate. It has been found necessary to add cellobiase from Aspergillus niger (NOVO) to the cellobiase component of Trichoderma reesei mutant Rut C-30 (Natick) cellulase enzymes in order to obtain after 48 hr complete conversion of the cellobiose formed in the enzymatic hydrolysis of biomass. This study of the cellobiase activity of these two enzyme sources was undertaken as a first step in the formation of a kinetic model for cellulose hydrolysis that can be used in process design. In order to cover the full range of cellobiose concentrations, it was necessary to develop separate kinetic parameters for high- and low-concentration ranges of cellobiose for the enzymes from each organism.

引用

页码：463 / 470

页数：8

共 9 条

[1]

ALLEN D, 1983, THESIS DARTMOUTH COL

[2]

GONG CS, 1977, BIOTECHNOL BIOENG, V19, P959, DOI 10.1002/bit.260190703

[3]

GROUS W, THESIS THAYER SCH EN

[4] COMBINED PRODUCT AND SUBSTRATE-INHIBITION EQUATION FOR CELLOBIASE [J].