MEMBRANE BILAYER ASSEMBLY IN NEURAL TISSUE OF RAT AND SQUID AS A CRITICAL PHENOMENON - INFLUENCE OF TEMPERATURE AND MEMBRANE-PROTEINS

被引:14
作者
GINSBERG, L [1 ]
GILBERT, DL [1 ]
GERSHFELD, NL [1 ]
机构
[1] NINCDS,BIOPHYS LAB,BETHESDA,MD 20892
关键词
BILAYER ASSEMBLY; NEURAL MEMBRANES; CRITICAL TEMPERATURE;
D O I
10.1007/BF01868541
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell membrane bilayers have been reconstructed in vitro utilizing total lipid extracts from rat neural tissue (forebrain, cerebellum, brainstem and spinal cord) and from the optic lobe and fin nerve of the squid Loligo pealei. In agreement with the critical state theory of bilayer assembly (Gershfeld, N.L. 1986. Biophys. J. 50:457-461: Gershfeld, N.L. 1989. J. Phys. Chem. 93:5256-5261), these lipid extracts spontaneously formed purely unilamellar structures in aqueous dispersion, but only at a critical temperature, T*, which was species dependent. For all the rat tissues T* = 37 +/- 1-degrees-C: for squid neural extracts T* = 15.5 +/- 1.4-degrees-C. These values correspond to 'physiological' temperatures for both organisms, implying that their lipid metabolism is geared to permit spontaneous assembly of unilamellar membranes at the ambient temperature in the tissues. Membrane protein composition had little or no effect on critical bilayer formation.
引用
收藏
页码:65 / 73
页数:9
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