MEMBRANE BILAYER ASSEMBLY IN NEURAL TISSUE OF RAT AND SQUID AS A CRITICAL PHENOMENON - INFLUENCE OF TEMPERATURE AND MEMBRANE-PROTEINS

被引：14

作者：

GINSBERG, L ^{[1
]}

GILBERT, DL ^{[1
]}

GERSHFELD, NL ^{[1
]}

机构：

[1] NINCDS,BIOPHYS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF MEMBRANE BIOLOGY | 1991年 / 119卷 / 01期

关键词：

BILAYER ASSEMBLY; NEURAL MEMBRANES; CRITICAL TEMPERATURE;

D O I：

10.1007/BF01868541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell membrane bilayers have been reconstructed in vitro utilizing total lipid extracts from rat neural tissue (forebrain, cerebellum, brainstem and spinal cord) and from the optic lobe and fin nerve of the squid Loligo pealei. In agreement with the critical state theory of bilayer assembly (Gershfeld, N.L. 1986. Biophys. J. 50:457-461: Gershfeld, N.L. 1989. J. Phys. Chem. 93:5256-5261), these lipid extracts spontaneously formed purely unilamellar structures in aqueous dispersion, but only at a critical temperature, T*, which was species dependent. For all the rat tissues T* = 37 +/- 1-degrees-C: for squid neural extracts T* = 15.5 +/- 1.4-degrees-C. These values correspond to 'physiological' temperatures for both organisms, implying that their lipid metabolism is geared to permit spontaneous assembly of unilamellar membranes at the ambient temperature in the tissues. Membrane protein composition had little or no effect on critical bilayer formation.

引用

页码：65 / 73

页数：9

共 40 条

[21] PHOSPHOLIPID-SYNTHESIS IN THE SQUID GIANT-AXON - INCORPORATION OF LIPID PRECURSORS [J].

GOULD, RM ;

PANT, H ;

GAINER, H ;

TYTELL, M .

JOURNAL OF NEUROCHEMISTRY, 1983, 40 (05) :1293-1299

[22] SYNTHESIS AND TURNOVER OF CEREBROSIDES AND PHOSPHATIDYLSERINE OF MYELIN AND MICROSOMAL FRACTIONS OF ADULT AND DEVELOPING RAT-BRAIN [J].

HAYES, LW ;

JUNGALWALA, FB .

BIOCHEMICAL JOURNAL, 1976, 160 (02) :195-204

[23] SYNTHESIS AND TURNOVER OF MEMBRANE PROTEIN AND LIPID IN MYCOPLASMA LAIDLAWII [J].

KAHANE, I ;

RAZIN, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 183 (01) :79-+

[24] INTERFERENCE BY LIPIDS IN THE DETERMINATION OF PROTEIN USING BICINCHONINIC ACID [J].

KESSLER, RJ ;

FANESTIL, DD .

ANALYTICAL BIOCHEMISTRY, 1986, 159 (01) :138-142

[25] TEMPERATURE-DEPENDENCE OF MOVEMENT OF POTASSIUM AND CHLORIDE-IONS ASSOCIATED WITH NERVE IMPULSES [J].

LANDOWNE, D ;

SCRUGGS, V .

JOURNAL OF PHYSIOLOGY-LONDON, 1976, 259 (01) :145-158

[26] INCORPORATION OF LABELLED PHOSPHATE INTO PHOSPHOLIPIDS IN SQUID GIANT AXONS [J].

LARRABEE, MG ;

BRINLEY, FJ .

JOURNAL OF NEUROCHEMISTRY, 1968, 15 (07) :533-&

[27]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[28] MEMBRANE SYNTHESIS IN BACILLUS-SUBTILIS .2. INTEGRATION OF MEMBRANE PROTEINS IN ABSENCE OF LIPID SYNTHESIS [J].

MINDICH, L .

JOURNAL OF MOLECULAR BIOLOGY, 1970, 49 (02) :433-&

[29] On the distribution of critical temperatures for spawning and for ciliary activity in bivalve molluscs [J].

Nelson, TC .

SCIENCE, 1928, 67 :220-221

[30]

Nicol J.A.C., 1960, BIOL MARINE ANIMALS

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