ATRIAL-NATRIURETIC-FACTOR RECOGNIZES 2 RECEPTOR SUBTYPES IN ENDOTHELIAL-CELLS CULTURED FROM BOVINE PULMONARY-ARTERY

被引：21

作者：

REDMOND, EM ^{[1
]}

CAHILL, PA ^{[1
]}

KEENAN, AK ^{[1
]}

机构：

[1] NATL UNIV IRELAND UNIV COLL DUBLIN,DEPT PHARMACOL,FOSTER AVE,DUBLIN 4,IRELAND

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

ANF receptor subtype; Endothelial cell; Pulmonary artery;

D O I：

10.1016/0014-5793(90)81143-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study specific high affinity binding sites for atrial natriuretic factor (rANF(99-126)) have been identified on cultured endothelial cells of bovine pulmonary artery origin (BPAEC). A time-dependent rise in cellular cGMP levels stimulated by rANF(99-126) was followed by release of the nucleotide into the incubation medium. The use of truncated, ring-deleted and linear atrial peptide analogs in competitive displacement analysis and measurement of cGMP accumulation indicated that only a minor proportion (5-11%) of the available receptor pool was of the ANF-B receptor subtype, linked to guanylate cyclase, with the remaining major proportion possibly of the ANF-C (clearance) receptor subtype. The existence of two ANF receptor subtypes in this cell culture model would suggest a significant role for the circulating peptide in modulation of pulmonary endothelial cell function, which would influence or complement its direct actions on the underlying vasculature of the pulmonary circulation. © 1990.

引用

页码：157 / 162

页数：6

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