CONSTITUTIVE EXPRESSION OF THE ID1 GENE IMPAIRS MOUSE B-CELL DEVELOPMENT

被引：252

作者：

SUN, XH

机构：

[1] Department of Cell Biology Kaplan Comprehensive Cancer Center New York Univ. Medical Center New York

来源：

CELL | 1994年 / 79卷 / 05期

关键词：

D O I：

10.1016/0092-8674(94)90078-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Id proteins are inhibitors of the basic-helix-loop-helix (bHLH) transcription factors. In the B cell lineage, the Id1 and Id2 genes are expressed in pro-B cells and down-regulated during differentiation. To determine the role of bHLH proteins and the significance of downregulation of ld genes in B cell development, transgenic mice constitutively expressing the Id1 gene were generated. Their phenotype suggests that B cell development is impaired at an early stage. Primarily, these mice have few B220(+) IgM(+) mature B or B220(+) CD43(-) pre-B cells in the bone marrow, reduced frequencies of V(D)J and V(kappa)J(kappa) recombination of the immunoglobulin loci, and lower expression levels of the immunoglobulin, RAG-1, RAG-2, and lambda 5 genes.

引用

页码：893 / 900

页数：8

共 57 条

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