DETERMINATION OF AMINO-ACIDS BY ONLINE CAPILLARY ELECTROPHORESIS-ELECTROSPRAY IONIZATION MASS-SPECTROMETRY

被引:42
作者
LU, W [1 ]
YANG, G [1 ]
COLE, RB [1 ]
机构
[1] UNIV NEW ORLEANS,DEPT CHEM,NEW ORLEANS,LA 70148
关键词
CAPILLARY ELECTROPHORESIS; ELECTROSPRAY IONIZATION; AMINO ACIDS;
D O I
10.1002/elps.1150160180
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
On-line capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESMS) has been used for the separation and detection of amino acid mixtures. Four natural amino acids, histidine, tryptophan, phenylalanine, aspartic acid, and a tripeptide, glutathione, were separated and determined. Protonated molecules were detected in the CE-ESMS mode with detection limits of about one pmol. Optimum CE-ESMS operating conditions for amino acid analysis were determined employing acetic acid solutions as CE electrolytes. The examined parameters included capillary diameter (50-100 mu m internal diameter), applied separation voltage (20-30 kV), and concentration of electrolyte (10-60% acetic acid). Stable working conditions were maintained when the CE currents were less than 18 mu A. The use of electrolyte solutions such as those described here instead of true buffer solutions may have advantages for CE-ESMS systems which employ a ''sheathless'' interface.
引用
收藏
页码:487 / 492
页数:6
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