GENE TRAP TAGGING OF PROLIFERA, AN ESSENTIAL MCM2-3-5-LIKE GENE IN ARABIDOPSIS

被引：208

作者：

SPRINGER, PS ^{[1
]}

MCCOMBIE, WR ^{[1
]}

SUNDARESAN, V ^{[1
]}

MARTIENSSEN, RA ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB, LITA ANNENBERG HAZEN GENOME CTR, COLD SPRING HARBOR, NY 11724 USA

来源：

SCIENCE | 1995年 / 268卷 / 5212期

关键词：

D O I：

10.1126/science.7754372

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megagametophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.

引用

页码：877 / 880

页数：4

共 48 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] HETEROLOGOUS TRANSPOSON TAGGING OF THE DRL1 LOCUS IN ARABIDOPSIS [J].

BANCROFT, I ;

JONES, JDG ;

DEAN, C .

PLANT CELL, 1993, 5 (06) :631-638

[3] DEVELOPMENT OF AN EFFICIENT 2-ELEMENT TRANSPOSON TAGGING SYSTEM IN ARABIDOPSIS-THALIANA [J].

BANCROFT, I ;

BHATT, AM ;

SJODIN, C ;

SCOFIELD, S ;

JONES, JDG ;

DEAN, C .

MOLECULAR AND GENERAL GENETICS, 1992, 233 (03) :449-461

[4] CHLOROPLAST GENE-EXPRESSION IN NUCLEAR, PHOTOSYNTHETIC MUTANTS OF MAIZE [J].

BARKAN, A ;

MILES, D ;

TAYLOR, WC .

EMBO JOURNAL, 1986, 5 (07) :1421-1427

[5] P-ELEMENT-MEDIATED ENHANCER DETECTION - A VERSATILE METHOD TO STUDY DEVELOPMENT IN DROSOPHILA [J].

BELLEN, HJ ;

OKANE, CJ ;

WILSON, C ;

GROSSNIKLAUS, U ;

PEARSON, RK ;

GEHRING, WJ .

GENES & DEVELOPMENT, 1989, 3 (09) :1288-1300

[6] SEARCHING FOR PATTERN AND MUTATION IN THE DROSOPHILA GENOME WITH A P-LACZ VECTOR [J].

BIER, E ;

VAESSIN, H ;

SHEPHERD, S ;

LEE, K ;

MCCALL, K ;

BARBEL, S ;

ACKERMAN, L ;

CARRETTO, R ;

UEMURA, T ;

GRELL, E ;

JAN, LY ;

JAN, YN .

GENES & DEVELOPMENT, 1989, 3 (09) :1273-1287

[7] A ROLE FOR THE NUCLEAR-ENVELOPE IN CONTROLLING DNA-REPLICATION WITHIN THE CELL-CYCLE [J].

BLOW, JJ ;

LASKEY, RA .

NATURE, 1988, 332 (6164) :546-548

[8] A 12.8 KB SEGMENT, ON THE RIGHT ARM OF CHROMOSOME-II FROM SACCHAROMYCES-CEREVISIAE INCLUDING PART OF THE DUR1,2 GENE, CONTAINS 5 PUTATIVE NEW GENES [J].

BUSSEREAU, F ;

MALLET, L ;

GAILLON, L ;

JACQUET, M .

YEAST, 1993, 9 (07) :797-806

[9]

Carlson W. R., 1988, Corn and corn improvement. Third edition., P259

[10]

Cone K., 1989, Maize Genetics Cooperation News Letter, P68

← 1 2 3 4 5 →