POLYMER-SUPPORTED BIO-POLYMER SYNTHESIS .5. ULTRAHIGH LOAD SOLID (GEL) PHASE PEPTIDE-SYNTHESIS - THE STEPWISE ELABORATION OF QUASI-HOMOGENEOUS PEPTIDE GEL NETWORKS

被引:7
作者
EPTON, R [1 ]
MARR, G [1 ]
MCGINN, BJ [1 ]
SMALL, PW [1 ]
WELLINGS, DA [1 ]
WILLIAMS, A [1 ]
机构
[1] WOLVERHAMPTON POLYTECH, SCH APPL SCI, WULFRUNA ST, WOLVERHAMPTON WV1 1LY, ENGLAND
关键词
D O I
10.1016/0141-8130(85)90027-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An approach to ultra-high load solid (gel) phase peptide synthesis is described in which a bead-form phenolic core polymer, crosslinked poly[N-{2-(4-hydroxyphenyl)ethyl}-acrylamide], is used as a support matrix at near theoretical maximum loading. Consecutive repeating units of the core polymer carry peptide chains undergoing stepwise elongation. Synthesis proceeds through a series of solvated networks, which consists mainly of protected peptide. The solvated networks are deemed to be quasi-homogeneous, insofar as each has a regular covalent framework and each is believed to be uniformly distributed throughout the gel beads. Illustrative synthesis of two fully-protected acylpeptide hydrazide segments, corresponding to dynorphin (6-12) and to .beta.h-endorphin (18-26), are described.
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页码:289 / 298
页数:10
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