MAPPING RNA REGIONS IN EUKARYOTIC RIBOSOMES THAT ARE ACCESSIBLE TO METHIDIUMPROPYL-EDTA-CENTER-DOT-FE(II) AND EDTA-CENTER-DOT-FE(II)

被引：31

作者：

HAN, H

SCHEPARTZ, A

PELLEGRINI, M

DERVAN, PB

机构：

[1] UNIV SO CALIF, PROGRAM MOLEC BIOL, LOS ANGELES, CA 90089 USA

[2] CALTECH, DIV CHEM & CHEM ENGN, PASADENA, CA 91125 USA

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 33期

关键词：

D O I：

10.1021/bi00199a004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methidiumpropyl-EDTA.Fe(II) [MPE.Fe(II)] and EDTA.Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE.Fe(II) and EDTA.Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.

引用

页码：9831 / 9844

页数：14

共 54 条

[21] COMPLEMENTARY OLIGODEOXYNUCLEOTIDE PROBES OF RNA CONFORMATION WITHIN THE ESCHERICHIA-COLI SMALL RIBOSOMAL-SUBUNIT [J].