ISOLATION, PURIFICATION AND CHARACTERIZATION OF BETA-HCGRP FROM HUMAN SPINAL-CORD

被引：32

作者：

WIMALAWANSA, SJ ^{[1
]}

MORRIS, HR ^{[1
]}

ETIENNE, A ^{[1
]}

BLENCH, I ^{[1
]}

PANICO, M ^{[1
]}

MACINTYRE, I ^{[1
]}

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL, DEPT BIOCHEM, LONDON SW7 2AZ, ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(90)90621-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human β-calcitonin gene-related peptide (β-hCGRP) was isolated and purified from spinal cord. The complete characterization of this material was based on definitive mass analysis by fast atom bombardment mass spectrometry together with gas phase sequencing. Combining these data we have characterized the structure of β-hCGRP including the C-terminal sequence, the presence of the S-S bridge and of phenylalanineamide as the C-terminal amino acid, and fully confirmed the amino acid sequence predicted from the nucleotide analysis. © 1990.

引用

页码：993 / 1000

页数：8

共 24 条

[1] THE CALCITONIN-LIKE SEQUENCE OF THE BETA-CGRP GENE
ALEVIZAKI, M
SHIRAISHI, A
RASSOOL, FV
FERRIER, GJM
MACINTYRE, I
LEGON, S
[J]. FEBS LETTERS, 1986, 206 (01): : 47 - 52
[2] ALTERNATIVE RNA PROCESSING IN CALCITONIN GENE-EXPRESSION GENERATES MESSENGER-RNAS ENCODING DIFFERENT POLYPEPTIDE PRODUCTS
AMARA, SG
JONAS, V
ROSENFELD, MG
ONG, ES
EVANS, RM
[J]. NATURE, 1982, 298 (5871) : 240 - 244
[3] CALCITONIN CALCITONIN GENE-RELATED PEPTIDE TRANSCRIPTION UNIT - TISSUE-SPECIFIC EXPRESSION INVOLVES SELECTIVE USE OF ALTERNATIVE POLYADENYLATION SITES
AMARA, SG
EVANS, RM
ROSENFELD, MG
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (10) : 2151 - 2160
[4] EXPRESSION BRAIN OF A MESSENGER-RNA ENCODING A NOVEL NEUROPEPTIDE HOMOLOGOUS TO CALCITONIN GENE-RELATED PEPTIDE
AMARA, SG
ARRIZA, JL
LEFF, SE
SWANSON, LW
EVANS, RM
ROSENFELD, MG
[J]. SCIENCE, 1985, 229 (4718) : 1094 - 1097
[5] RAPID METHOD, USING OCTADECASILYL-SILICA, FOR EXTRACTION OF CERTAIN PEPTIDES FROM TISSUES
BENNETT, HPJ
HUDSON, AM
KELLY, L
MCMARTIN, C
PURDON, GE
[J]. BIOCHEMICAL JOURNAL, 1978, 175 (03) : 1139 - 1141
[6] PURIFICATION AND CHARACTERIZATION OF A PEPTIDE FROM AMYLOID-RICH PANCREASES OF TYPE-2 DIABETIC-PATIENTS
COOPER, GJS
WILLIS, AC
CLARK, A
TURNER, RC
SIM, RB
REID, KBM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) : 8628 - 8632
[7] GIBSON SJ, 1984, J NEUROSCI, V4, P3101, DOI 10.1523/jneurosci.04-12-03101.1984
[8] GIRGIS SI, 1985, LANCET, V2, P14
[9] HOPPENER JWM, 1985, HUM GENET, V70, P259
[10] LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 3 →