STEROID 21-HYDROXYLASE ACTIVITY IN EQUINE OVARIAN FOLLICLES EVIDENCED BY ISOTOPE DILUTION-MASS SPECTROMETRY

被引：5

作者：

BIJAULT, C ^{[1
]}

DEHENNIN, L ^{[1
]}

机构：

[1] FDN RECH HORMONOL,F-94260 FRESNES,FRANCE

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 38卷 / 02期

关键词：

D O I：

10.1016/0960-0760(91)90122-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steroid 21-hydroxylase activity of the microsome-enriched fraction of follicular linings from equine ovaries has been demonstrated by gas chromatography-mass spectrometry. The 21-hydroxylated metabolites were quantified by isotope dilution with deuterated analogues. The two most abundant potential substrates for follicular steroid 21-hydroxylase, progesterone (P) and 17-hydroxyprogesterone (17OHP), were converted respectively to 11-deoxycorticosterone (DOC) and 11-deoxycortisol with corresponding apparent specific activities of 308 and 24 pmol/mg protein/h and apparent K(m) values of 1.1 and 6.4-mu-M. Competitive inhibition of the P-to-DOC conversion was exerted by 17OHP and pregnenolone. Hence, the ovarian follicle of the mare is an extraadrenal site of preferential DOC biosynthesis by an enzyme having steroid 21-hydroxylase activity.

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页码：165 / 172

页数：8

相关论文

共 30 条

[1] STEROIDAL CYCLIC KETALS .12. THE PREPARATION OF DELTA-16-STEROIDS [J].

ALLEN, WS ;

BERNSTEIN, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1955, 77 (04) :1028-1032

[2] THE ORIGIN OF PLASMA DEOXYCORTICOSTERONE IN MEN AND IN WOMEN DURING THE MENSTRUAL-CYCLE [J].

ANTONIPILLAI, I ;

MOGHISSI, E ;

HAWKS, D ;

SCHNEIDER, T ;

HORTON, R .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1983, 56 (01) :93-98

[3] ORIGIN OF 5ALPHA-PREGNANE-3ALPHA,20ALPHA,21-TRIOL 3-SULFATE IN PREGNANT-WOMEN [J].

BAILLIE, TA ;

SJOVALL, J ;

SJOVALL, K .

FEBS LETTERS, 1975, 60 (01) :145-148

[4] CONVERSION OF PROGESTERONE TO DEOXYCORTICOSTERONE IN THE HUMAN-FETUS - STEROID 21-HYDROXYLASE ACTIVITY IN FETAL TISSUES [J].

CASEY, ML ;

WINKEL, CA ;

MACDONALD, PC .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1983, 18 (04) :449-452

[5]

CASEY ML, 1985, ANN NY ACAD SCI, V458, P2342

[6]

CHASALOW FI, 1979, J BIOL CHEM, V254, P3777

[7] STRUCTURE OF A BOVINE GENE FOR P-450C21 (STEROID 21-HYDROXYLASE) DEFINES A NOVEL CYTOCHROME-P-450 GENE FAMILY [J].

CHUNG, BC ;

MATTESON, KJ ;

MILLER, WL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4243-4247

[8] BIOSYNTHESIS OF 11-DEOXYCORTICOSTEROIDS BY TELEOST OVARIES AND DISCUSSION OF THEIR POSSIBLE ROLE IN OOCYTE MATURATION AND OVULATION [J].

COLOMBO, L ;

BERN, HA ;

PIEPRZYK, J ;

JOHNSON, DW .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1973, 21 (01) :168-178

[9] ANDROGEN AND 19-NORSTEROID PROFILES IN HUMAN PREOVULATORY FOLLICLES FROM STIMULATED CYCLES - AN ISOTOPE-DILUTION MASS-SPECTROMETRIC STUDY [J].

DEHENNIN, L ;

JONDET, M ;

SCHOLLER, R .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1987, 26 (03) :399-405

[10] ESTROGENS, ANDROGENS, AND PROGESTINS IN FOLLICULAR-FLUID FROM PREOVULATORY FOLLICLES - IDENTIFICATION AND QUANTIFICATION BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY ASSOCIATED WITH STABLE ISOTOPE-DILUTION [J].

DEHENNIN, L .

STEROIDS, 1990, 55 (04) :181-184