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ISOLATION OF A BACILLUS-SUBTILIS MUTANT DEFECTIVE IN CONSTITUTIVE O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE

被引：4
作者：
MOROHOSHI, F
MUNAKATA, N
机构：
[1] Radiobiology Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, 104
来源：
MUTATION RESEARCH | 1990年 / 235卷 / 01期
关键词：
Alkylating agent; Bacillus subtilis; N-Methyl-N′-nitro-N-nitrosoguanidine; O[!sup]6[!/sup]-alkylguanine-DNA alkyltransferase;
D O I：
10.1016/0921-8777(90)90018-Z
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A mutant of Bacillus subtilis defective in the constitutive activity of O6-alkylguanine-DNA alkyltransferase was isolated from a strain (ada-1) deficient in the adaptive response to DNA alkylation. Cells carrying the mutation dat-1 which was responsible for the defect in constitutive activity exhibited hypersensitivity for lethality and mutagenesis when challenged with methyl-nitroso compounds. The constitutive activity is independent of the adaptive response, and seems to function as a basal defense against environmental alkylating agents. © 1990.
引用
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页码：15 / 23
页数：9
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