ISOLATION OF A BACILLUS-SUBTILIS MUTANT DEFECTIVE IN CONSTITUTIVE O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE

被引：4

作者：

MOROHOSHI, F

MUNAKATA, N

机构：

[1] Radiobiology Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, 104

来源：

MUTATION RESEARCH | 1990年 / 235卷 / 01期

关键词：

Alkylating agent; Bacillus subtilis; N-Methyl-N′-nitro-N-nitrosoguanidine; O[!sup]6[!/sup]-alkylguanine-DNA alkyltransferase;

D O I：

10.1016/0921-8777(90)90018-Z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A mutant of Bacillus subtilis defective in the constitutive activity of O6-alkylguanine-DNA alkyltransferase was isolated from a strain (ada-1) deficient in the adaptive response to DNA alkylation. Cells carrying the mutation dat-1 which was responsible for the defect in constitutive activity exhibited hypersensitivity for lethality and mutagenesis when challenged with methyl-nitroso compounds. The constitutive activity is independent of the adaptive response, and seems to function as a basal defense against environmental alkylating agents. © 1990.

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页码：15 / 23

页数：9

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