LIPOSOME FLOW-INJECTION IMMUNOASSAY - MODEL-CALCULATIONS OF COMPETITIVE IMMUNOREACTIONS INVOLVING UNIVALENT AND MULTIVALENT LIGANDS

被引:31
作者
YAP, WT
LOCASCIOBROWN, L
PLANT, AL
CHOQUETTE, SJ
HORVATH, V
DURST, RA
机构
[1] Center for Analytical Chemistry, NIST, Gaithersburg
关键词
D O I
10.1021/ac00018a020
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The use of liposomes as detectable reagents in solid-phase immunoassays has been explored in a flow injection immunoanalysis (FIIA) system. Model calculations are presented for FIIA based on the competitive binding of univalent analyte and multivalent liposomes to immobilized antibodies. Parameters such as binding constants, concentrations of liposomes and antibody, and steric hindrance are considered for their relative effects on detectable liposome signal response to analyte concentrations. Qualitative comparisons of the model with the experimental data are made.
引用
收藏
页码:2007 / 2011
页数:5
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