MONOCLONAL-ANTIBODY ANTI-MPO IS USEFUL IN RECOGNIZING MINIMALLY DIFFERENTIATED ACUTE MYELOID-LEUKEMIA

被引：14

作者：

PRAXEDES, MK ^{[1
]}

DEOLIVEIRA, LZ ^{[1
]}

PEREIRA, WD ^{[1
]}

QUINTANA, IZ ^{[1
]}

TABAK, DG ^{[1
]}

DEOLIVEIRA, MSP ^{[1
]}

机构：

[1] INST NACL CANC,CEMO,CELL MARKERS LAB,PRACA CRUZ VERMELHA 23,RIO JANEIRO,BRAZIL

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 12卷 / 3-4期

关键词：

MONOCLONAL ANTIBODY; ANTI-MPO; MINIMALLY DIFFERENTIATED LEUKEMIA;

D O I：

10.3109/10428199409059594

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The enzyme myeloperoxidase (MPO) is the most specific marker of myeloid lineage. The recognition of acute myeloid leukaemia (AML) with minimally differentiation (AML-M0) is established with methods that include myeloid markers CD13/CD33 and detection of MPO in blast cells by immunological techniques or electron microscopy Cytochemistry (EM). We have analysed the presence of MPO in leukaemic blast cells by conventional cytochemistry and immunological methods using a monoclonal antibody anti-MPO (CLB-MPO1) in 121 cases of acute leukaemia. The aim of the study was to investigate the sensitivity of this McAb to identify AML-MO, as CD13/CD33 can be expressed in some cases of acute lymphoblastic leukaemia (ALL) and EM cytochemistry is not always available in many laboratories. Anti-MPO was positive in all cases of AML (MI-M5) which were positive by Sudan Black B reaction in similar or higher percentage ratio for each case, although in some of them did not label with CD13/CD33 tested by IF and IPc techniques. Based on the anti-MPO positivity, 5 out of 10 cases called undifferentiated leukaemia (AUL), were reclassified as AML-MO, though 4 cases were CD13/CD33 negative. Furthermore, after analysing the anti-MPO expression among 32 cases of ALL, we had to reclassify four of them as acute biphenotypic leukaemia. We conclude that anti-MPO is a very sensitive and reliable tool in AML diagnosis and has an important role in distinguishing minimally differentiated AML and biphenotypic acute leukaemia from AUL and ALL.

引用

页码：233 / 239

页数：7

共 25 条

[11]

Moreschita Y., Biochemical characterization of human myeloperoxidase using three specific monoclonal antibodies, Brit. J. Haematol., 63, pp. 435-444, (1986)

[12]

Storr J., Dolan G., Coustain-Smith E., Barnett D., Reilly R.T., Value of monoclonal anti-myeloperoxidase (MPO7) for diagnosing acute leukaemia, J. Clin. Pathol., 43, pp. 847-849, (1990)

[13]

Van der Schoot C.E., Daams G.M., Pinkster J., Vet R., Von dem Borne A.E.G., Monoclonal antibodies against myeloperoxidase are valuable immunological reagents for the diagnosis of acute myeloid leukaemias, Brit. J. Haematol., 74, pp. 173-178, (1990)

[14]

Buccheri V., Shetty V., Yoshida N., Morilla R., Matutes E., Catovsky D., The role of an anti-myeloperoxidase antibody in the diagnosis and classification of acute leukaemia: a comparison with light and electron microscopy cytochemistry, Brit. J. Haematol., 80, pp. 62-68, (1992)

[15]

Dacie J.V., Lewis S.M., Catovsky D., Dacie J.V., Lewis S.M., Blood cell cytochemistry and supplementary techniques, Practical Haematol, pp. 84-94, (1984)

[16]

Bennett J., Catovsky D., Daniel M.T., Flandrin G., Gal-Ton D.A.G., Gralnick H.R., Sultan C., Proposal for the classification of the acute leukaemias (FAB cooperative group), Brit. J. Haematol., 33, pp. 451-458, (1976)

[17]

Bennett J., Catovsky D., Daniel M.T., Flandrin G., Galton D.A.G., Gralnick H.R., Sultan C., Criteria for the diagnosis of acute leukaemia of megakaryocyte lineage (M7). A report of the French-American-British Cooperative group, Ann. Intern. Med., 103, pp. 460-462, (1985)

[18]

Chan L.C., Pelgram S.M., Greaves M.F., Contribution of immunophenotyping to the classification and differential diagnosis of acute leukaemia, Lancet, 1, pp. 475-479, (1985)

[19]

Janossy G., Counstain-Smith E., Campana D., The reliability of cytoplasmic CD3 and CD22 antigen expression in the immunodiagnosis of acute leukemia—a study of 500 cases, Leukemia, 3, pp. 170-181, (1989)

[20]

Scott C.S., Vulliamy T., Catovsky D., Matutes E., Norfolk D.R., DNA genotypic conservation during phenotypic switch from T-cell acute lymphoblastic leukaemia to acute myeloblastic leukaemia, Leukemia and Lymphoma, 1, pp. 21-28, (1989)

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