GENETIC-STUDIES IN MARINE FISHES .2. A PROTEIN ELECTROPHORETIC ANALYSIS OF POPULATION-STRUCTURE IN THE RED DRUM SCIAENOPS-OCELLATUS

Nine polymorphic loci were found among 42 presumptive protein-coding gene loci surveyed among 474 red drum (Sciaenops ocellatus) sampled in 1987 from 13 nearshore and 1 offshore localities from the Atlantic coast of the southeastern USA and the northern Gulf of Mexico. The mean number of alleles over the polymorphic loci was 3.8, and the average heterozygosity over all loci examined (HBAR) was estimated as 0.047. These data indicate that red drum have "normal" levels of genetic variability. Wright's F(ST) values (the standardized variance of allele frequencies between samples) over all polymorphic loci ranged from 0.009 to 0.027 (mean F(ST) = 0.019), and estimates of the effective number of migrants (N(e)m) per generation using Wright's island model ranged from 9.0 to 27.5. High levels of gene flow among the red drum samples were also indicated by Slatkin's qualitative analysis using conditional average allele frequencies. Nei's estimates of genetic distance between pairs of samples ranged from 0.000 to 0.009, indicating a high degree of nuclear gene similarity among all samples. Highly significant heterogeneity in allele frequencies at the locus for adenosine deaminase was detected between red drum sampled from the Atlantic and those sampled from the Gulf and among red drum sampled from the Gulf.