QUENCHING OF SINGLET OXYGEN BY BIOMOLECULES FROM L1210 LEUKEMIA-CELLS

被引：98

作者：

BAKER, A

KANOFSKY, JR

机构：

[1] EDWARD HINES JR DEPT VET AFFAIRS HOSP, MED SERV, HINES, IL 60141 USA

[2] LOYOLA UNIV, STRITCH SCH MED, DEPT MED, MAYWOOD, IL 60153 USA

[3] LOYOLA UNIV, STRITCH SCH MED, DEPT BIOCHEM, MAYWOOD, IL 60153 USA

[4] EDWARD HINES JR DEPT VET AFFAIRS HOSP, RES SERV, HINES, IL 60141 USA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1992年 / 55卷 / 04期

关键词：

D O I：

10.1111/j.1751-1097.1992.tb04273.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Singlet oxygen lifetimes for detergent-dispersed L1210 leukemia cells in deuterium oxide buffer were measured by following the decay of 1270 nm phosphorescence. Four photosensitizers and two detergents were studied. Stern-Volmer plots were linear over the cell concentration range studied (0-10(7) cells/mL). The singlet-oxygen quenching constants obtained depended somewhat upon the specific combination of detergent and photosensitizer used. Extrapolation of the singlet-oxygen lifetime data to "100%" cell concentration (1.39 +/- 0.04 x 10(9) cells/mL) and correction for the contribution of the water solvent gave a singlet-oxygen lifetime between 0.17 and 0.32-mu-s for the L1210 leukemia cell. The theoretical contributions of various types of biological molecules within the L1210 cell to the total singlet-oxygen quenching were calculated from their concentrations and their quenching constants. These calculations suggest that proteins will quench most of the singlet-oxygen. Only about 7% of the singlet-oxygen is quenched by water.

引用

页码：523 / 528

页数：6