EWS-FLI-1 AND EWS-ERG CHIMERIC MESSENGER-RNAS IN EWINGS-SARCOMA AND PRIMITIVE NEUROECTODERMAL TUMOR

被引：44

作者：

IDA, K

KOBAYASHI, S

TAKI, T

HANADA, R

BESSHO, F

YAMAMORI, S

SUGIMOTO, T

OHKI, M

HAYASHI, Y

机构：

[1] UNIV TOKYO,FAC MED,DEPT PEDIAT,BUNKYO KU,TOKYO 113,JAPAN

[2] SAITAMA CHILDRENS MED CTR,DIV HEMATOL ONCOL,IWATSUKI,SAITAMA,JAPAN

[3] MITSUBISHI KAGAKU BIOCLIN LABS INC,GENE ANAL LAB,TOKYO,JAPAN

[4] MIYAZAKI MED UNIV,DEPT PEDIAT,MIYAZAKI,JAPAN

[5] NATL INST CANC RES,DIV RADIOBIOL,TOKYO,JAPAN

来源：

INTERNATIONAL JOURNAL OF CANCER | 1995年 / 63卷 / 04期

关键词：

D O I：

10.1002/ijc.2910630407

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The t(11;22)(q24;q12) and t(21;22)(q22;q12) are specific chromosomal translocations found in the Ewing family of tumors including ES, PNET and Askin tumors. In these translocations, the amino-terminal portion of the EWS gene located in 22q12 fuses to the carboxyl-terminal portion of the FLI-1 gene located in 11q24 or the ERG gene located in 21q22, which belong to the ets oncogene superfamily of transcription activators. We investigated the chimeric mRNAs of 15 ESs (7 cell liner and 8 tumor samples) and 7 PNETs (3 cell lines and 4 tumor samples) using the RT-PCR method and sequencing. We detected 2 types of EWS-ERG chimeric mRNA in 2 ES cell lines and PNET tumor sample in addition to 4 types of EWS-FLI-1 chimeric mRNA in 11 ESs (4 cell lines and 7 tumor samples) and 4 PNETs (2 cell lines and 2 tumor samples). There seemed to be no association between the type of chimeric mRNA and clinical features such as sex, age, primary site and histopathology of the patients. All of the chimeric mRNAs are generated from in-frame junctions and are thought to encode fusion proteins that may be the molecular mechanism involved in the Ewing family of tumors. (C) 1995 Wiley-Liss, Inc.

引用

页码：500 / 504

页数：5

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