TRANSCRIPTIONAL ANALYSIS OF THE STREPTOMYCES-GLAUCESCENS TETRACENOMYCIN-C BIOSYNTHESIS GENE-CLUSTER

被引：24

作者：

DECKER, H

HUTCHINSON, CR

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 12期

关键词：

D O I：

10.1128/JB.175.12.3887-3892.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.

引用

页码：3887 / 3892

页数：6

共 44 条

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