TRANSCRIPTIONAL ANALYSIS OF THE STREPTOMYCES-GLAUCESCENS TETRACENOMYCIN-C BIOSYNTHESIS GENE-CLUSTER

被引：24

作者：

DECKER, H

HUTCHINSON, CR

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 12期

关键词：

D O I：

10.1128/JB.175.12.3887-3892.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.

引用

页码：3887 / 3892

页数：6

共 44 条

[21] TTA CODONS IN SOME GENES PREVENT THEIR EXPRESSION IN A CLASS OF DEVELOPMENTAL, ANTIBIOTIC-NEGATIVE, STREPTOMYCES MUTANTS
LESKIW, BK
LAWLOR, EJ
FERNANDEZABALOS, JM
CHATER, KF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) : 2461 - 2465
[22] THE USE OF A RARE CODON SPECIFICALLY DURING DEVELOPMENT
LESKIW, BK
BIBB, MJ
CHATER, KF
[J]. MOLECULAR MICROBIOLOGY, 1991, 5 (12) : 2861 - 2867
[23] IDENTITY OF CO-SYNTHETIC FACTOR 1 OF STREPTOMYCES-AUREOFACIENS AND FRAGMENT FO FROM CO-ENZYME F420 OF METHANOBACTERIUM SPECIES
MCCORMICK, JRD
MORTON, GO
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (14) : 4014 - 4015
[24] ISOLATION OF TETRACENOMYCIN-C-NONPRODUCING STREPTOMYCES-GLAUCESCENS MUTANTS
MOTAMEDI, H
WENDTPIENKOWSKI, E
HUTCHINSON, CR
[J]. JOURNAL OF BACTERIOLOGY, 1986, 167 (02) : 575 - 580
[25] MOTAMEDI H, UNPUB
[26] MOTAMEDI H, 1987, GENET IND MICROORGAN, P355
[27] BIDIRECTIONAL PROMOTER AND TERMINATOR REGIONS BRACKET MMR, A RESISTANCE GENE EMBEDDED IN THE STREPTOMYCES-COELICOLOR A3(2) GENE-CLUSTER ENCODING METHYLENOMYCIN PRODUCTION
NEAL, RJ
CHATER, KF
[J]. GENE, 1991, 100 : 75 - 83
[28] OPTIMIZATION OF GENE-EXPRESSION IN STREPTOMYCES-LIVIDANS BY A TRANSCRIPTION TERMINATOR
PULIDO, D
JIMENEZ, A
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (10) : 4227 - 4240
[29] RAIBAUD AM, J BACTERIOL, V173, P4454
[30] RHODES PM, 1981, J GEN MICROBIOL, V124, P329

← 1 2 3 4 5 →