STRUCTURAL AND DEVELOPMENTAL ANALYSIS OF A GENE CLONED FROM THE EARLY ECDYSTERONE-INDUCIBLE PUFF SITE, I-18C, IN CHIRONOMUS-TENTANS

被引：7

作者：

DORSCHHASLER, K ^{[1
]}

LUTZ, B ^{[1
]}

SPINDLER, KD ^{[1
]}

LEZZI, M ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST ZOOL,W-4000 DUSSELDORF 1,GERMANY

来源：

GENE | 1990年 / 96卷 / 02期

关键词：

alternative splicing; developmental regulation; Northern blots; primer extension; Recombinant DNA; S1; nuclease;

D O I：

10.1016/0378-1119(90)90258-S

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A gene (I-18C) cloned from the early ecdysterone (EDS)-inducible puff site, I-18C, in Chironomus tentans salivary glands, codes by differential processing for at least five transcripts which can be grouped into spliced and unspliced transcripts. The spliced group comprises several RNAs of approx. 4.6 kb, and the unspliced group comprises the 1.8-kb and 6.5-kb RNAs. All these transcripts have a similar or the same transcription start point. The steady-state level of the spliced 4.6-kb RNAs reveals some parallels with EDS concentrations in animals during the various developmental stages analyzed, while the levels of the unspliced 1.8-kb and 6.5-kb RNAs do not correlate with the EDS concentrations. © 1990.

引用

页码：233 / 239

页数：7

共 20 条

[1] COMPLETE DNA-SEQUENCE OF THE ECDYSTERONE-CONTROLLED GENE I-18C OF CHIRONOMUS-TENTANS [J].

AMREIN, K ;

LUTZ, B ;

HERTNER, T ;

LEZZI, M ;

DORSCHHAESLER, K .

NUCLEIC ACIDS RESEARCH, 1986, 14 (19) :7807-7808

[2] 2 TRANSCRIPTS OF THE SAME ECDYSTERONE-CONTROLLED GENE ARE DIFFERENTIALLY ASSOCIATED WITH RIBOSOMES [J].

AMREIN, K ;

DORSCHHASLER, K ;

LUTZ, B ;

LEZZI, M .

GENE, 1988, 65 (02) :277-283

[3] PUFFS, GENES, AND HORMONES REVISITED [J].

ASHBURNER, M .

CELL, 1990, 61 (01) :1-3

[4] THE DROSOPHILA 74EF EARLY PUFF CONTAINS E74, A COMPLEX ECDYSONE-INDUCIBLE GENE THAT ENCODES 2 ETS-RELATED PROTEINS [J].

BURTIS, KC ;

THUMMEL, CS ;

JONES, CW ;

KARIM, FD ;

HOGNESS, DS .

CELL, 1990, 61 (01) :85-99

[5] SYNTHESIS OF SPERM AND LATE HISTONE CDNAS OF THE SEA-URCHIN WITH A PRIMER COMPLEMENTARY TO THE CONSERVED 3' TERMINAL PALINDROME - EVIDENCE FOR TISSUE-SPECIFIC AND MORE GENERAL HISTONE GENE VARIANTS [J].

BUSSLINGER, M ;

BARBERIS, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (17) :5676-5680

[6]

DREESEN TD, 1985, J BIOL CHEM, V260, P1824

[7] MICROCLONING OF A LONG SEGMENT OF A GENE FROM THE PRIMARY ECDYSTERONE-CONTROLLED PUFF REGION I-18C OF CHIRONOMUS-TENTANS - MOLECULAR CHARACTERIZATION AND EFFECT OF HORMONE ON ITS TRANSCRIPTION [J].

HERTNER, T ;

HERTNERMEYER, B ;

SOGO, JM ;

SCHLAPFER, G ;

JACKLE, H ;

EDSTROM, JE ;

LEZZI, M .

DEVELOPMENTAL BIOLOGY, 1986, 113 (01) :29-39

[8] DETERMINATION OF THE DEVELOPMENTAL STAGE OF LIVING 4TH INSTAR LARVAE OF CHIRONOMUS-TENTANS [J].

INEICHEN, H ;

MEYER, B ;

LEZZI, M .

DEVELOPMENTAL BIOLOGY, 1983, 98 (02) :278-286

[9] THE 93D HEAT-SHOCK LOCUS OF DROSOPHILA-MELANOGASTER - MODULATION BY GENETIC AND DEVELOPMENTAL FACTORS [J].

LAKHOTIA, SC .

GENOME, 1989, 31 (02) :677-683

[10] ECDYSTEROID TITERS IN CHIRONOMUS AND THEIR RELATION TO HEMOGLOBINS AND VITELLOGENINS [J].

LAUFER, H ;

VAFOPOULOUMANDALOS, X ;

DEAK, P .

INSECT BIOCHEMISTRY, 1986, 16 (01) :281-285

← 1 2 →